In view of the poor adaptability of microbial activated water flooding to Sai169 low-permeability reservoir, Changqing Oilfield, the types and contents of metabolites such as biosurfactants, organic acids and biogas in microbially activated water in the on-field expanding culture insert were quantified by gas chromatography-mass spectrometry and other testing methods, the mechanism of micro oil displacement was clarified, and the microbial community distribution in the produced fluid of 22 wells was sequenced by the paired-end sequencing method. The results of the study showed that the mass concentration of esters in the microbial metabolites ranged from 1.57 to 4.98 mg/L, the mass concentration of organic acids ranged from 108.34 to 133.61 mg/L, the volume concentration of biogas ranged from 18 to 38 mL/L, the oil-water interfacial tension was reduced by 30% to 70%, the pH value was reduced by 3% to 5%, and the oil displacement efficiency was increased by 5.46 to 11.93 percentage points; the biosurfactants, organic acids, biogas and biopolymers in the microbially activated water metabolites played an important role in oil displacement; the gene numbers of bacteria, archaea and methanogens in the fluids of production wells generally increased in the process of microbial flooding, and the differences in distribution between wells gradually decreased; the differences in microbial communities between wells were mainly related to permeability and injection-production horizons. This study provides an important theoretical basis for microbial flooding, formation and well selection, and optimization of injection-production scheme in low-permeability heterogeneous reservoir.
Zhuang Jian
,
Lu Canyang
,
Zhang Xu
,
Yang Wenjun
,
Yuan Weibin
. Mechanism and Community Distribution of Microbially Activated Water Flooding in Sai 169 Low-permeability Reservoir[J]. Special Oil & Gas Reservoirs, 2022
, 29(1)
: 147
-153
.
DOI: 10.3969/j.issn.1006-6535.2022.01.022
[1] 黄曼,刘倩倩.微生物在采矿及石油开采中的应用[J],科技资讯,2011,2(22):99-101.
HUANG Man,LIU Qianqian.The application of microorganisms in mining and petroleum exploitation[J].Science & Technology Information,2011,2(22):99-101.
[2] LI Guoqiang,GAO Peike,WU Yunqiang,et al.Microbial abundance and community composition influence production performance in a low-temperature petroleum reservoir[J].Environmental Science & Technology,2014,48(9):5336-5344.
[3] 袁自学,王靖云,李淑珣,等.特低渗透注水砂岩油藏采收率确定方法[J].石油勘探与开发,2014,41(3):341-348.
YUAN Zixue,WANG Jingyun,LI Shuxun,et al.A new approach to estimating recovery factor for extra-low permeability water-flooding sandstone reservoir[J].Petroleum Exploration and Development,2014,41(3):341-348.
[4] GUDINA E J,PEREIRA F B,COSTA R,et al.Biosurfactant-producing and oil-degrading bacillus subtilis strains enhance oil recovery in laboratory sand-pack columns[J].Journal of Hazardous Materials,2013,261(15):106-113.
[5] 刘骊川.微生物驱油技术的研究进展与应用前景[J].中外能源,2009,14(3):41-45.
LIU Lichuan.Advances in researching the techniques of microbial enhanced oil recovery and their application prospects[J].Sino-Global Energy,2009,14(3):41-45.
[6] SINGH A,PARMAR N,WARD O.Biotechnology in enhanced petroleum oil recovery[J].Geomicrobiology and Biogeochemistry,2014,10(39):239-245.
[7] ROBERT Thomas,ANBU Clemensis,JOHNSONB Robert,et al.Biotechnology in the petroleum industry:an overview[J].International Biodeterioration & Biodegradation,2014,86(1):225-237.
[8] 黄立信.典型油藏微生物群落解析及驱油机理研究[D].北京:中国科学院研究生院(渗流流体力学研究所),2014.
HUANG Lixin.Analysis of microbial communities in typical oil reservoirs and study of oil displacement mechanism[D].Beijing:University of Chinese Academy of Sciences(Institute of Porous Flow and Fluid Mechanics),2014.
[9] 徐婷,佘跃惠,何延龙,等.微生物驱矿场采出液中生物代谢产物[J].大庆石油地质与开发,2020,39(2):107-113.
XU Ting,SHE Yuehui,HE Yanlong,et al.Microbial metabolites in the produced liquid for MEOR field[J].Petroleum Geology & Oilfield Development in Daqing,2020,39(2):107-113.
[10] 韩春春,佘跃惠,张凡,等.长庆油田延9低渗透油藏微生物群落研究[J].生物技术,2012,22(1):67-72.
HAN Chunchun,SHE Yuehui,ZHANG Fan,et al.The microbial communities of low permeability Yan9 Reservoir in Changqing Oilfield[J].Biotechnology,2012,22(1):67-72.
[11] 国家能源局.微生物驱油技术规范:SY/T 6888—2012[S].北京:石油工业出版社,2012:1.
National Energy Administration.Microbial flooding technical specifications:SY/T 6888-2012[S].Beijing:Petroleum Industry Press,2012:1.
[12] 王大威,刘永建,杨振宇,等.脂肽生物表面活性剂在微生物采油中的应用[J].石油学报,2008,29(1):111-115.
WANG Dawei,LIU Yongjian,YANG Zhenyu,et al.Application of surfactin in microbial enhanced oil recovery[J].Acta Petrolei Sinica,2008,29(1):111-115.
[13] 于娟,陈爱华,马天玲,等.三次采油石油微生物及代谢产物分析评价方法[J].新疆石油天然气,2008,4(4):88-91.
YU Juan,CHEN Aihua,MA Tianling,et al.Analytic method of oil microbes and their metablizing substances in the third oil recovery[J].Xinjiang Oil & Gas,2008,4(4):88-91.
